Spatially resolved transcriptomics datasets prepared from different publications to be used as sample data sets for the SSAM-lite tool. Each folder contains a dataset including: one file for mRNA locations, formatted as a comma separated file adhering to the DecodedSpots format defined by the Starfish pipeline (https://spacetx-starfish.readthedocs.io/en/mcai-api-additions/help_and_reference/spacetx-format/output_formats/DecodedSpots/index.html). This format is intuitive, with fields for the target mRNA, the x-coordinate and the y-coordinate. a gene signature file, containing expected mRNA expression profiles for cell types, formatted as a comma separated file with rows as cell types, columns as genes, with the matrix populated with numerical or binary values associating mRNA expression to cell types. a csv for a custom color palette for the provided cell types. and a README referencing the original publication the data was retrieved from as well as some dataset specifications. Datsets specifications: Codeluppi_osmFISH method: osmFISH tissue: mouse brain somatosensory cortex mRNA spots: 1802589 genes: 33 signatures: 31 cell types DOI: 10.1038/s41592-018-0175-z Tosti_ISS_Pancreas method: ISS tissue: human pancreas mRNA spots: 461078 genes: 138 signatures: 16 cell types DOI: 10.1053/j.gastro.2020.11.010