Transgenic mice expressing the calcium-sensitive fluorescent indicator GCamp6f were trained in the Accumulating Towers task and two-photon calcium imaging was performed of functionally identified primary and secondary visual cortical areas, as well as retrosplenial cortex. Regions of interest representing putative single neurons were extracted using a semi-customized demixing and deconvolution procedure. Behavioral data were separately collected by a computer that ran a virtual reality control engine, and synchronized with imaging data via a synchronization timestamp sent to the imaging computer at every graphics refresh cycle.

Funding provided by: National Institutes of HealthCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000002Award Number: 5U01NS090541Funding provided by: National Institutes of HealthCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000002Award Number: 1U19NS104648

Condensed neural and behavioral data, included secondary results from model fits and analyses. This dataset contains all information required to recreate figures from the paper.