Traditional analytical approaches, including high performance liquid chromatography (HPLC), gas chromatography (GC), and ultraviolet (UV) detection, cannot effectively address the emerging problems of species specificity and sensitivity analysis. A modern analytical technique called transferring is depend upon the traditional use of specific detection of molecules or elements in electrophoresis or chromatography. Currently, the most common methods for the estimation of trace elements include a combination of separation methods and more sensitive detection methods. Previously, this binding method was an additional combination of specialized offline sample preparation, separation and detection methods. Scripting technology is currently being developed by combining separation technology (chromatography) and spectroscopic detection technology online. The method written combines chromatographic and spectral methods to use both. Chromatography allows you to obtain pure or almost pure fractions of chemical components in a mixture. Spectroscopy uses standard spectra or libraries to obtain selective information for identification. This technology offers faster analysis times, higher automation, better throughput, better reproducibility, and less pollution. Since it is a closed system, it provides better binding selectivity and more information. The tremendous progress in written analytical methods over the last two decades has greatly expanded their application in the analysis of biomaterials, especially natural products. This article discusses the latest advances in various technologies such as GCMS, LC-MS, LC-NMR, and CE-MS. As part of preliminary analysis of raw extracts or fractions from various sources, online isolation and discovery of natural substances, and chemical taxonomic studies.