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ZENODO
Dataset . 2021
License: CC BY
Data sources: Datacite
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ZENODO
Dataset . 2021
License: CC BY
Data sources: Datacite
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
ZENODO
Dataset . 2021
License: CC BY
Data sources: ZENODO
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ZENODO
Dataset . 2021
License: CC BY
Data sources: Datacite
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ZENODO
Dataset . 2021
License: CC BY
Data sources: ZENODO
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Claim

Analysis of RNA polymerase II phosphorylation in two-color STED microscopy images

Research datakeyboard_double_arrow_right Dataset 17 Jun 2021Publisher:Zenodo
Authors: Hilbert, Lennart;

doi: 10.5281/zenodo.4972490 , 10.5281/zenodo.4972491 , 10.5281/zenodo.5229952

Analysis of RNA polymerase II phosphorylation in two-color STED microscopy images

Abstract

This data set includes the raw image data and MatLab analysis scripts to assess the relative distribution of RNA polymerase II C-terminal domain serine 5 and serine 2 phosphorylation in zebrafish embryos. Phosphorylated polymerase II was labeled by immunofluorescence, microscopy images were acquired by STED microscopy and analyzed using MatLab scripts. This extended version now contains image data and analysis scripts to two alternative sets of antibodies.

Steps needed to carry out the analysis: 1. Install bfmatlab from OME, add it to the MatLab path 2. Extract the image data by running the script STED_extraction_lif.m 3. Analyze the extracted data by running the script ClusterAnalysis.m 4. Generate example images by running the script ExampleImages.m

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