Objectives: The current study aimed to investigate the association between the use of hormonal contraceptives and development of vaginal candidiasis infection. Methods: Vaginal swabs were collected from 270 women after inclusion and exclusion criteria, of which, 202 women were contraceptive users and 68 women were not using contraceptives. Detailed clinical history and full examination including vaginal examination were done. After clinical diagnosis has been made, appropriate laboratory tests for isolation of Candida and its species were done via using the standard procedures. Results: Out of 270 women, 145 (53.7%; %95 CI: 40.4- 52.2) were found to have positive Candida culture with Candida albicans was the dominant species isolated. vaginal candidiasis was more prevalent among contraceptive users as compared to not using contraceptives (84.1% vs. 15.9%) respectively (P<0.001). All Candida species were found significantly more prevalent among contraceptive users and Candida albicans identified to have the higher proportion (P< 0.05). Vaginal candiasis was significantly more prevalent among women who using (COCP) than those who using injectable or (IUCD) (90.16% vs. 6.56% vs. 3.28%) respectively, with significant prevalence differences of its species regarding contraceptive methods used (P< 0.001). Conclusion: A strong association have been found between the prevalence of vaginal candidiasis and its Candida species and the use of contraceptives. Among contraceptive methods, the Combined Oral Contraceptive Pill (COCP) is the most influencing method that contributes to the vaginal candidiasis.