Data underlying the figures in the publication “In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism”, published in ACS Chem. Biol., 2021, 16, 2, 397–403. https://pubs.acs.org/doi/10.1021/acschembio.0c00968 Table of contents: 1. Table 1; Text document containing the Michaelis - Menten kinetic data for thiourocaate hydratase, obtained with UV-Vis spectrophotometer as described in the supporting information of the publication. 2. Figure 3; Text document containing the numerical data for Figure 3: Reverse phase HPLC traces (wavelength 220 nm) for each metabolite in ergothioneine degradation pathway. 3. Figure 4; Data and model validation report of X-ray crystallography diffraction data of thiouricanate hydratase collected at the swiss light source at 100K. Data processed and refined according to standard procedure.