RNAi of the Drosophila mitochondrial fusion genes Opa1 and Marf (Mitofusin 2) in the muscle results in an extended lifespan. In order to unravel the metabolic changes in the long-living flies we analysed the metabolome by NMR spectroscopy, comparing the two knock-down genotypes to a wild type control at two ages: 30 days (young flies) and 65 days (old flies). In order to detect autonomous and non-autonomous changes, we also divided the samples in three anatomical regions: head (mostly neural tissue) thorax (mostly muscle), and abdomen (visceral, reproductive, metabolic control)

Work was funded by PROMETEU/2018/135 from "Consellería, de Sanitat de la Generalitat Valenciana". Part of the equipment employed in this work has been funded by Generalitat Valenciana and co-financed with ERDF funds (OP ERDF of Comunitat Valenciana 2014–2020).