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Deciphering the low abundance microbiota of presumed aseptic hip and knee implants This data set includes input files and results associated with our work "Deciphering the low abundance microbiota of presumed aseptic hip and knee implants" (currently under review). Contents aitch_dist.txt: Aitchison distance matrix contam_by_freq_0.2.txt: decontam results (identifying contaminants by frequency) contam_by_prev_0.2.txt: decontam results (identifying contaminants by prevalence) counts.txt: counts table resulting from reads processed with custom demultiplexing script cutadapt_counts.txt: counts table resulting from reads processed with cutadapt cutadapt_meta.txt: metadata table accompanying cutadapt_counts.txt cutadapt_tax.txt: taxonomy table resulting from reads processed with cutadapt cutadapt_track.txt: table summarizing retention of reads processed with cutadapt throughout the DADA2 pipeline dna_concs.txt: DNA concentrations per sample extraction_aldex2.txt: ALDEx2 results for comparison of DNA extraction methodologies extraction_envfit_and_pca.RData: envfit results and PCA biplot for DNA extraction methodologies extraction_shannon_diversity.txt: Shannon diversity and extraction methodology per sample fwd_barcodes.fasta: forward barcodes for use by cutadapt meta.txt: metadata table accompanying counts.txt meta_st.txt: metadata for use by SourceTracker pt_oac_aldex2.txt: ALDEx2 results for comparison of sample type, controlling for the effect of DNA extraction methodology pt_oac_envfit_and_pca.RData: envfit results and PCA biplot for sample type pt_oac_shannon_diversity.txt: Shannon diversity, sample type, and extraction methodology per sample rev_barcodes.fasta: reverse barcodes for use by cutadapt spike_1_dists.txt: Aitchison distances between spike 1 samples and other samples on the same plate spike_2_dists.txt: Aitchison distances between spike 1 samples and other samples on the same plate tax.txt: taxonomy table resulting from reads processed with custom demultiplexing script track.txt: table summarizing retention of reads processed with custom demultiplexing script throughout the DADA2 pipeline Code Availability Scripts to process and produce these data are available at https://github.com/charlie-carr/implant_microbiota Citation The full citation will be updated after the manuscript is published.
Low abundance microbiota, Total knee arthroplasty, Total hip arthroplasty, Infection, 16S rRNA sequencing
Low abundance microbiota, Total knee arthroplasty, Total hip arthroplasty, Infection, 16S rRNA sequencing
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