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These are the data that are related to the manuscript "Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor" by Eike K. Mahlandt1,*, Janine J. G. Arts1,2,Werner J. van der Meer1, Franka H. van der Linden1, Simon Tol2, Jaap D. van Buul1,2, Theodorus W. J. Gadella Jr.1, Joachim Goedhart1,* 1 Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands 2 Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands
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