Hormone estrogen and progesterone is one of its compounds a steroid in characterization by a different behaviour. Estrogen functions in accordance with sex hormones women and act as main hormone in women for progesterone normally produced by men and women. The purpose of this research is to know the hormones estrogen and progestron in mice models ovariektomi in therapy using flour deproteinasi madidihang bony fishes, non deproteinasi and calcium carbonate as a control. Research methodology used to research this is the method his experiments with Kit ELISA readers with a wavelength 450 nm. This research result indicates that therapy flour bones of fish madidihang non deproteinasi with doses 1600 mg/kg BB/day on that therapy flour bones of fish madidihang non deproteinasi with doses 1600 mg/kg BB/day on in mice model ovariektomi