To conduct a mixture risk assessment, it is necessary to estimate exposure levels to bisphenol A not associated with observable risks to semen quality in men of reproductive age, so-called reference doses. To our knowledge, previous systematic reviews of the association between bisphenol A have only considered epidemiological evidence (Bliatka et al. 2020; Bonde et al. 2017) and there is therefore a need to update and complement these works. To support this effort, a systematic review of associations between bisphenol A exposures and declining semen quality is required, both from human and from animal studies. This publication is a detailed protocol for this systematic review effort.