Polysome profile analysis of human pancreatic beta-cells exposure to glucose A human pancreatic beta cell line (betaH2) was exposed to glucose after starvation. Total RNA was collected for a bulk RNA-seq analysis. Three biological repeats were produced for each of the the low and high glucose conditions resulting in to 6 sequence (fastq) files. Three different mRNA-ribosomal fractions were selected from a sucrose gradient corresponding to single ribosomes (monosomes), light ribosomes (2-4) and heavy ribosomes (5 and more). Three biological repeats were produced for each fraction and for the two conditions (low and high glucose) resulting to 18 different (fastq) sequenced files. In total the study consists of 24 submitted fastq files, 6 corresponding to total RNA from two conditions (three replicates) and 18 corresponding to polysome profile, 3 fractions times two conditions times 3 replicates. All 24 *.fastq.gz files are available in the reads_PolysomeProfile_RNAseq_BetaCells.tar archive. In addition to the raw reads we provide the processed data: The raw counts table ( countsTOTALS_CodingGenes.tsv) and the Transcripts Per Million table (TPMs) (polysomeProfile_TPM_proteinCoding.csv) files of the data produced from the polysome profile and RNA-seq experiments described in the experimental design that can be found in: https://github.com/parisepigenetics/Translatome_Bcells_glucose