Data underlying the figures in the publication “Synchronized reagent delivery in double emulsions for triggering chemical reactions and gene expression”, published in Small Methods. Table of contents: 1. Figure 2C; Origin file containing all data and analysis for Figure 2C. Requires Origin Software to open. 2. Figure 3; FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure 3B and 3C. Requires FlowJo software to open. Raw data file names: 200721 FDG in PVA after prod_FDG sample 6_006.fcs 200721 FDG in PVA&SDS 4 h after prod_FDG sample 4 PVA shake_002.fcs 200721 FDG in PVA&SDS 4 h after prod_FDG sample 4 SDS 001 shake_004.fcs 200721 FDG in PVA&SDS 22 h after prod_FDG sample 4 PVA shake_002.fcs 200721 FDG in PVA&SDS 22 h after prod_FDG sample 4 SDS 001 shake_004.fcs 3. Figure 4BC; Origin file containing all data and analysis for Figure 4B and 4C. Requires Origin Software to open. 4. Figure 5; Origin file containing all data and analysis for Figure 5D. Requires Origin Software to open. File Figure 5_201215-new LUVs 500 mM DH5alpha: FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure 5B. Requires FlowJo software to open. Raw data file names: 201215-new LUVs 500 mM-DH5alpha_sample 1 18h after prod_012.fcs 201215-new LUVs 500 mM-DH5alpha_sample 3 18h after prod- 05%SDS_018.fcs 5. Figure S2; PDF files, report from DLS instrument. 6. Figure S4; Excel file containing the values extracted from microscopy images for Figure S4. 7. Figure S5; FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure S5. Requires FlowJo software to open. Raw data file names: 200721 FDG in PVA after prod_FDG sample 6_006.fcs 200721 FDG in PVA&SDS 4 h after prod_FDG sample 4 SDS 001 shake_004.fcs 200721 FDG in PVA&SDS 4 h after prod_FDG sample 5 SDS 01 shake_008.fcs 200721 FDG in PVA&SDS 4 h after prod_FDG sample 6 SDS 0001 shake_006.fcs 200721 FDG in PVA&SDS 22 h after prod_FDG sample 4 SDS 001 shake_004.fcs 200721 FDG in PVA&SDS 22 h after prod_FDG sample 5 SDS 01 shake_008.fcs 200721 FDG in PVA&SDS 22 h after prod_FDG sample 6 SDS 0001 shake_006.fcs 8. Figure S6; Origin file containing all data and analysis for Figure S6 left-Requires Origin Software to open-, and Excel file containing the values from 96 well plate measurements used for Figure S6 right. 9. Figure S7; Excel files containing the values from 96 well plate measurements used for Figure S7 left, and the values extracted from microscopy images for Figure S7 right. 10. Figure S9; FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure S9. Requires FlowJo software to open. Raw data file names: 201215-new LUVs 500 mM-DH5alpha_sample 1 3h30min after prod_005.fcs 201215-new LUVs 500 mM-DH5alpha_sample 1 18h after prod_012.fcs 201215-new LUVs 500 mM-DH5alpha_sample 1 30min after prod_001.fcs 201215-new LUVs 500 mM-DH5alpha_sample 3 3h30min after prod - 05%SDS_008.fcs 201215-new LUVs 500 mM-DH5alpha_sample 3 18h after prod- 05%SDS_018.fcs