The goal of this work was to determine the suitability of selected blood biomarkers of articular cartilage as mechanosensitive markers and to investigate the dose-response relationship between ambulatory load magnitude and marker kinetics in response to load. Serum samples were collected from 24 healthy volunteers before (0) and at three time points (1=0min; 2=30min; 3=60min) after a 30-minute walking stress test performed on a treadmill on three test days. In each experimental session, one of three ambulatory loads was applied: 100% body weight (BW); 80%BW; 120%BW. Serum concentrations of COMP, MMP-3, MMP-9, ADAMTS-4, PRG-4, CPII, C2C and IL-6 were assessed using commercial enzyme-linked immunosorbent assays. ADAMTS - A disintegrin and metalloproteinase with thrombospondin motifs C2C - COL2−3/4Clong mono epitope COMP - cartilage oligomeric matrix protein CPII - C-propeptide is removed from the procollagen extracellularly and directly reflects the rate of type II procollagen synthesis IL - interleukin MMP - matrix metalloproteinasis PRG - proteoglycan The file contains the raw concentrations for each marker, participant, time point and condition.

Raw data as text files can be obtained upon request from the author.