This protocol describes a rapid DNA and RNA extraction from plant tissues as a handmade kit. Hexadecyltrimethylammonium bromide (CTAB), Sodium chloride (NaCl), Tris base, and Ethylenediaminetetraacetic acid (EDTA) are the main components of this extraction buffer. In contrast to previous works and all reported protocols, the required components of this protocol have established without providing any stock solutions and it will be prepared just by adding them directly. This isolation buffer can extract both DNA and RNA simultaneously. Depending on the purpose of our experiment, RNA and DNA can be selected. Forever, extracting DNA and RNA from plant tissues in a short time by using general laboratory equipment and reach to the high quality of nucleic acid are noticeable. This method probably is usable for all plant tissues and takes only one hour.