Objective: The purpose of this study was to determine the bacteriological aetiology and incidence of Urinary Tract Infections (UTIs) and antibacterial susceptibility patterns among pregnant women attending antenatal clinics at the Federal Medical Centre in Bida, Niger-State, using the gold standard of culture. Study Design: Data were obtained from the Medical Microbiology Department Registry from pregnant women who attended routine antenatal clinics between January 2010 and December 2012.A total of 1242 pregnant women aged 15 to 54 years old had mid-stream urine samples taken. At the first antenatal appointment, samples were collected inside sterile disposable universal bottles from pregnant women suspected of having UTIs. SPSS version 16.0 was used to code, compute, and analyse the data, and p values of 0.05 were considered statistically significant. Results: Out of 1242 pregnant women between the ages of 15 and 54 in this study, our research found that the incidence of UTI was 46.1 percent, which was statistically significant (p = 0.046, with a mean age of 30.92 years and a standard deviation of 5.46). The most common uropathogen (60%) was Escherichia coli, followed by Staphylococcus aureus (28.3%). Escherichia coli was susceptible to Nitrofurantoin and Gentamycin (61.4%) and (51.5%) respectfully. Conclusion: The incidence of urinary tract infection in this study was 46.1% uropathogen, which is cause for concern among the general public. As a result, pregnant women should be screened for symptomatic or asymptomatic UTIs at antenatal clinics on a regular basis.