A new mygalomorph spider genus, Afropesa n. gen., is established for three South African species: the type species A. schoutedeni (Benoit, 1965) n. comb., transferred here from Entypesa Simon, 1902, and two newly described congeners, A. gauteng n. sp. and A. schwendingeri n. sp. The new genus differs from other genera of the Entypesidae by a unique set of diagnostic characters, including a flanged embolus and the spermathecae with wide bases and lengthened distal lobes. The three included species can be distinguished from each other by a shape of the male tibia and metatarsus I, as well as by the structure of the embolus and configuration of the spermathecae.