Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid Wei Gu, MD, PhD1,2*, Andreas M. Rauschecker, MD, PhD3, Elaine Hsu, BS1, Kelsey C. Zorn, BS4, Yasemin Sucu, BS1, Scot Federman, BS1, Allan Gopez, BS1, Shaun Arevalo, BS1, Hannah A. Sample, BS4, Eric Talevich, PhD5, Eric D. Nguyen, MD, PhD 1, Marc Gottschall, BS1, Bardia Nourbakhsh, MD, MAS6, Carl A. Gold, MD, MS7, Bruce A.C. Cree, MD, PhD, MAS8, Vanja Douglas, MD8, Megan B. Richie, MD8, Maulik P. Shah, MD, MHS8, S. Andrew Josephson, MD8, Jeffrey M. Gelfand, MD, MAS8, Steve Miller, MD, PhD 1, Linlin Wang, MD1, Tarik Tihan, MD, PhD9, Joseph L. DeRisi, PhD4,10, Charles Y. Chiu, MD, PhD11,12, Michael R. Wilson, MD, MAS8* 1Department of Laboratory Medicine, University of California San Francisco, CA 94107, USA 2Department of Pathology, Stanford University, CA 94305, USA 3Department of Radiology and Biomedical Imaging, University of California San Francisco, CA 94107, USA 4Department of Biochemistry and Biophysics, University of California San Francisco, CA 94107, USA 5DNANexus, Mountain View, CA 94040, USA 6Department of Neurology, Johns Hopkins University, MD 21287, USA 7Department of Neurology, Stanford University, CA 94305, USA 8Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, CA 94107, USA 9Department of Pathology, University of California San Francisco, CA 94107, USA 10Chan Zuckerberg Biohub, San Francisco, CA 94107, USA 11UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 91407, USA 12Department of Medicine, Division of Infectious Diseases, University of California San Francisco, CA 94107, USA * Corresponding authors: Michael Wilson, MD, MAS UCSF Department of Neurology 675 Nelson Rising Lane, NS212 San Francisco, CA 94158 Michael.Wilson@ucsf.edu Wei Gu, MD, PhD Stanford University, Department of Pathology 3373 Hillview Ave, 218 Palo Alto, CA 94304 650-723-1914 weigu@stanford.edu

v2 updates: Added a segment of files that were missed on the first upload. Added Dr. Bardia Nourbakhsh to the author list.