The enzyme URE 13-300 is an α-transglucosylase, part of GH70 family, synthesizing insoluble glucan with α-1,6 and α-1,3 linkage. This enzyme is an object of great interest because of the high prebiotic potential of its oligosaccharide products and branched glucan. The obtained enzyme was used in enzyme reactions for synthesis of insoluble glucan using sucrose as donor of glucose residues and maltose and low-molecular dextran as acceptors.

Acknowledgements: This work was supported by Department for Scientific Research – PU, Bulgaria, grant МУ19-БФ-019/2019, and by operational program "Science and education for smart growth" 2014–2020, grant number BG05M2OP001-1.002-0005-C01, Personalized Innovative Medicine Competence Centre (PERIMED).