The spontaneous tail coiling (STC) of zebrafish embryos represents the earliest observable motor activity in the developing neural network. We present here the STC data for single substances (singlechemicaldata) used for the concentration response curves which are published in the paper: "Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an automated workflow in KNIME by Ogungbemi et al. 2020. In addition, we present STC data for chemical mixtures (mixturedata) which were used to generate the concentration response curves in the paper manuscript: "Assessing combined effects for mixtures of similar and dissimilar acting neuroactive substances on zebrafish embryo movement by Ogungbemi et al. 2021 For more details on the data, please see the papers.