Drosophila pupa notum, captured with a laser-scanning confocal microscope (LSM 880, Zeiss). Pixel size 0.188 µm, z step 1 µm. This dataset also contains the parameters to generate the reference surface and local projection, also included. It is meant to be used with the Fiji plugin called 'LocalZProjector': https://gitlab.pasteur.fr/iah-public/localzprojector. The two *.localzprojector files can be loaded directly in the plugin to load adequate parameters. See the accompanying paper: DProj: A toolbox for local 2D projection and accurate morphometrics of large 3D microscopy images. https://www.biorxiv.org/content/10.1101/2021.01.15.426809v2 This is a downsampled version of the dataset used to generate the figures 1, 2 and 3 in this paper, as well as the comparison in table 1.