In this study, we used clownfish Amphiprion ocellaris as a model organism to study reef fish mechanisms of thermal adaptation and determine how high temperature affects different lipid aspects in fish. We exposed juvenile fish to two different experimental conditions, implemented over 28 days: average tropical water temperatures (26 ˚C, control) or average warm pool temperatures (30 ˚C). We then performed several analyses on fish muscle and liver tissues: i) total lipid content (%), ii) lipid peroxides, iii) fatty acid profiles, iv) lipid metabolic pathways and v) body condition. Raw data from these analyses were compiled and included in three datasheets: one for the fatty acid masses/concentration datasets, the second for body condition (length and weight) dataset and the third for fatty acid enrichment dataset based on LMSD – Lipid Maps Structure Database; KEGG – Kyoto Encyclopedia of Genes and Genomes; and HMBD – Human Metabolome Database. Statistical analyses and conclusions from the study can be found in Madeira et al. 2021 entitled "Conserved fatty acid profiles and lipid metabolic pathways in a tropical reef fish exposed to ocean warming – an adaptation mechanism of tolerant species?", published in STOTEN, https://doi.org/10.1016/j.scitotenv.2021.146738

This work was funded by: i) the Portuguese Fundação para a Ciência e Tecnologia (FCT) through the projects PTDC/MAR-EST/2141/2012, UIDB/04292/2020 granted to MARE, UIDB/04326/2020 granted to CCMAR and UIDB/04378/2020 granted to UCIBIO; ii) CESAM (UIDP/50017/2020+UIDB/50017/2020) by FCT/MEC through national funds, and the co-funding by FEDER, within PT2020 Partnership Agreement and Compete 2020; iii) work in the organic chemistry laboratory at Eawag Kastanienbaum was funded by internal Eawag funds. CM was supported by a researcher grant CEECIND/01526/2018 provided by FCT. D.M. was supported by a researcher grant CEECIND/01250/2018, by FCT. M.L. was supported by the Integrated Programme of SR&TD "SmartBioR - Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund.