Bicycle tourism is one of the new tourism trends that is not included in the main tourism markets, but has been growing and attracting tourists in recent years. In this respect, it provides an important opportunity to attract different tourist groups for tourism destinations. Understanding the demands and needs of this new tourism market is essential. The aim of this research is to determine the travel motivation of bicycle tourists. The research is thought to be important in determining the motivation factors of bicycle tourists and helping to determine tourism strategies according to these factors. Research data were obtained from Turkish tourists coming from outside the Canakkale city, who participated in the Bicycle Tour Festival held in Çanakkale between 24-25 March in 2018. Research data were obtained from 369 participants by using the survey technique. Explanatory and confirmatory factor analysis was applied to the data obtained from the participants. As a result of the research, cycling tourism motivations determined in five dimensions. These dimensions are named as discovering / relaxing nature; competence / mastery; new experience / knowledge and gaining dignity / socializing. When the second-level confirmatory factor analysis was done, the most effective dimensions in bicycle tourism motivation were competence / mastery, new experience / knowledge and socialization. The dimension in which the tourists participated at the highest level was the dimension of discovering nature and relaxation. The lowest level of participation was the dimension of gaining prestige.