Background: Organ donation is described as giving one or more organs, without compensation, for transplantation to another person. It is defined as a life-saving treatment of choice for multiple end-stage organ diseases. Our study's objective is to assess the knowledge, attitude, and practice of organ donation among AL-Dammam and AL-Khobar communities.Method: This was an analytical community-based cross-sectional study, conducted in two Eastern Province cities in Saudi Arabia. A convenient sample was approached by using a web-based questionnaire to collect data from the population. We included all who completely filled responses, reaching a total of 1179 participants. Data were compiled and analyzed using a statistical package for the social sciences (SPSS, version 16). Results: The study found that only 48.1% of cases agreed that organ transplants successfully prolong and improve a recipient's life quality. Almost half of the patients (49.9%) agreed that organ donation is vital to help those who need the organs. The majority of participants (73.3%) know that it is allowed to give or receive organs from a KSA living person. Social media was the most common source of knowledge about organ donation. Conclusion: Our study found reported good levels of awareness about organ donation among the participants, and most of them had a positive attitude towards organ donation. Keywords: Organ donation; Organ transplantation; Brain death; Deceased donor; Saudi Arabia.