Introduction: Neonatal resuscitation (NR) is a means to restore life to a baby from the state of asphyxia. It is a single intervention of birth asphyxia. Objectives: The main objectives of the study is to analyse the knowledge and experience of newborn resuscitation practices among nurses of Lahore. Material and methods: This descriptive study was conducted in The Children's Hospital and Institute of Child Health, Lahore during June 2019 to January 2020. Data were collected from the study subjects in a pre-selected comfortable setting and using a pre-tested face-to-face interview administered questionnaire. Results: The data was collected from 400 nurses of the Lahore from different hospitals. The mean age of the participants was 29.5±5.67 years. The study showed that only one-fourth (25%) of the respondents were taken additional training on neonatal resuscitation. Nearly half of the study participants had performed resuscitation at least once with the basic training they took in college before graduation, and had ever worked in the delivery room. Conclusion: It is concluded that nurses should be always prepared to deliver newborn resuscitation. Poor knowledge and poor skill regarding newborn resuscitation among nurses observed in the study.