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GitHub repository linked: sudu87/Microbial-ecology-of-the-transplanted-human-lung. This contains the code, bacterial culture collection and README.md. Linked to publication: https://doi.org/10.1101/2020.05.21.106211 Description of datasets given below: All_BAL_samples_raw_fastqc: FastQC reports for raw sequencing data after merging all samples. All_BAL_samples_processed_fastqc: FastQC reports for trimmed and curated merged data. Supplementary_Data_1: Table of 16S rRNA amplicon sequencing information, such as OTU relative abundance and taxonomy, by sample, without negative controls. Supplementary_Data_2: Table of 16S rRNA amplicon sequencing information, such as OTU relative abundance and taxonomy, for each negative control. Supplementary_Data_3: Detailed table showing frequency of all OTUs detected across the 234 BALF samples in terms of their abundance and prevalence. Supplementary_Data_4: List of Lung microbiota culture collection (LuMiCol) isolates with detailed information about sample number, culture conditions, taxonomy. This is a non-curated list containing about 300 isolates of which 215 were used for analysis. Supplementary_Data_5: OTU-isolate match summary listing the isolates that match OTUs in their 16S rRNA gene sequence, and information about the number of representative isolates in LuMiCol, with at least one representative isolate name, prevalence, and preference in terms of oxygen condition and media. Supplementary_Data_6: Detailed input and output files from unsupervised machine learning approach used by Genocrunch to determine Partition around medoids (PAMs) i.e. pneumotypes. The zip file includes parameters files: json and text files and Silhouette plots. Supplementary_Data_7: Detailed metadata table associated with all patients and samples.
{"references": ["A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation Sudip Das, Eric Bernasconi, Angela Koutsokera, Daniel-Adrien Wurlod, Vishwachi Tripathi, Germ\u00e1n Bonilla-Rosso, John-David Aubert, Marie-France Derkenne, Louis Mercier, C\u00e9line Pattaroni, Alexis Rapin, Christophe von Garnier, Benjamin J. Marsland, Philipp Engel, Laurent P. Nicod bioRxiv 2020.05.21.106211; doi: https://doi.org/10.1101/2020.05.21.106211"]}
lung ecology, lung transplant, bacterial culture, 16S amplicon sequencing, gene expression, machine learning, CLAD, anellovirus
lung ecology, lung transplant, bacterial culture, 16S amplicon sequencing, gene expression, machine learning, CLAD, anellovirus
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