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ZENODO
Dataset . 2021
License: CC BY
Data sources: ZENODO
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ZENODO
Dataset . 2021
License: CC BY
Data sources: Datacite
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Example data from timelapse fluorescence imaging experiments

Authors: Mahlandt, E.K.; Arts, J.J.G.; Goedhart, J;

Example data from timelapse fluorescence imaging experiments

Abstract

Example data from timelapse fluorescence imaging experiments. The zip file 0Archive_all_example_data.zip contains all the data: 201029_PMN_channel_06-2.tif: Migration of fluorescent leukocytes (label: Calcein red-orange). Time interval ~10s. EB3-timelapse.tif: Microtubule dynamics. Nocodazole is added midway through the movie to disrupt the microtubules (label: EB3-GFP). Time interval ~10s. mNeon-CAAX.tif: Plasma membrane dynamics of endothelial cells (label: mNeonGreen-CaaX). Time interval ~2s. 2010230_BOEC-mTq2-CaaX.tif: Morphological change of an endothelial cell due to contraction induced by thrombin (label: mTurquoise2-CaaX). Time interval ~40s. 210203_LEXY_combined.tif: Blue light activation of nuclear export (probe: LEXY tagged with mCherry). Time interval ~30s. Exposure with blue light at frames: 1-20,55-65,101-112,148-182 YCaM.zip: Calcium dynamics activated with histamine and deactivated by pyrilamine (probe: Yellow Cameleon 3.60, with Cyan and Yellow fluorescent protein). Time interval ~2s. Files and material for image processing and data analysis is available here: https://github.com/JoachimGoedhart/TimeLapse-Imaging-DataViz

Keywords

image analysis, cell biology, data visualization, timelapse imaging, fluorescence microscopy

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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