Aim: The aim was to evaluate physicians' and nurses' perceptions toward services provision at primary healthcare centers in Dammam and AlKhobar, Saudi Arabia. Methods: An analytical cross-sectional study at Ministry of health Primary Health care centers in Dammam and AL Khobar eastern province, Saudi Arabia. Thirteen (13) primary health care centers in AL Khobar and 30 centers in Dammam were included. A structured multi-item questionnaire was used to collect data from physicians and nurses about their perception toward workplace services as workforce, infrastructure, health care service, and teamwork barriers at primary healthcare centers. Results: Out of 364 of the participants, 94.2% were Saudi nationals, and 78.3% were female. About 51% of the studied sample were general practitioners, specialists, and consultants, while the rest were nurses (49%). The highest agreement score regarding the barriers at workforce were the unavailability of adequate social workers and dieticians, then lab technicians.; While for the infrastructures the unavailability of cafeteria services has the highest agreement score followed by the unavailability of the library, staff rest room, electronic medical records and comprehensive electronic healthcare system follow close behind it.; For the health care services, unavailability of ultrasound equipment had the highest percentage as a barrier, followed by the unavailability of X-ray equipment, essential clinical equipment, ECG machine, and lab services. most of of the participants were not aware of their job description and not satisfied with their colleague (83.2% and 76.9%, respectively). Conclusion: Physicians and nurses at primary health care centers in Dammam and ALKobar faced multiple barriers related to workforce, infrastructures and healthcare services. Which consequently affect the quality of health care provided by healthcare professional to targeted populations.