Aim: To assess serum lactate dehydrogenase 2 (LD2) levels in patients with NHL with and with-out bone marrow penetration. Introduction: Lactate dehydrogenase (LDH) is significantly increased in patients with haemopoietic cancer and has been shown to have prognostic value, especially in patients with non-Hodgkin's lymphoma. Serum LD2 is determined in 60 patients who have previously been diagnosed with NHL. Place and Duration: In the Oncology Department of Sir Ganga Ram Hospital Lahore for one-year duration from December 2018 to November 2019. Goals and Objectives: Patients were separated into 2 groups with 30 bone marrow infiltrates (group B) and were left without infiltration (group C). The values were equated with 20 healthy persons in terms of sex and age (group-A). Estimates were made before the chemotherapy initiation. Results: There was also a substantial difference among the values of NHL patients with & without bone-marrow infiltration. The levels showed a positive correlation with the spread of the disease.LD2 levels in NH2 patients increased significantly compared to controls. Conclusion: We find that the above-mentioned non-invasive parameter is a useful indicator of the extent of the disease. Key words: Hodgkin's disease, non-Hodgkin's lymphoma, lymphatic lymph tissue, lactate dehydrogenase.