Objective: To test and evaluate the accuracy and safety of an automatic biopsy gun for collecting kidney tissue samples in children. Design: prospective study in a hospital. Place and duration: Pediatric Medicine Unit, Bahawal Victoria Hospital, Bahawalpur for one-year duration from June 2019 to June 2020. Methodology: Fifty children diagnosed with nephrotic syndrome (steroid-dependent, steroid-resistant and frequently relapsing) underwent percutaneous ultrasound-guided kidney biopsy with an automatic biopsy gun (needle size 16G or 18G) under local anesthesia in 1-3 trials in each patient, followed by observation after biopsy for 24 hours. Results: With a majority of 38 men (76%), the age ranged from 2 to 14 years with a mean ± SD (7.20 ± 3.41) years. A suitable kidney tissue sample was obtained in 48 (96%) cases, while 2 (4%) tissue samples were inadequate. The core length of the tissue sample was mean ± SD (1.04 ± 0.37) and the number of glomeruli per tissue sample remained mean ± SD (13.07 ± 3.62). Five patients developed a small perirenal hematoma <2 cm. Conclusion: Percutaneous ultrasound-guided kidney biopsy in children is a fairly accurate and safe procedure. Observation for 24 hours allows you to identify any complications. Keywords: Kidney biopsy in children, automatic biopsy gun, safety, complications