Objective: The objective of this study case was to draw an analogy of frequency of gallstonesbetween patients diagnosed with Hepatitis C and those without the virus. Place and Duration of Study: The examination spanned almost two years (January 2018 – September 2018) at the Mayo hospital, Lahore. Materials and Methods:By using the Non probability sampling technique, 1000 cases were included, regardless of age and sex. The patients were examined by the gimmune chromatographic strip-test method for the Anti-HCV antibody.Individual groups for those with Hepatitis C and without it were categorized. Group 1 included seronegative while the Group 2 compromised of seropositive patients. The abdomen region was scanned with ultrasound to determine the presence of gallstones. A particular program was design for the collection of data of age, gender, absence or presence of gallstones. The details were assessed by employing SPSS V.21. Description statistics and Chi square test was implemented. ResultsAn aggregate number of 1000 patients were incorporated into the examination, which were classified into two groups. In Group I, 500 patients were HCV seronegative and keeping in mind that in Group II 500 patients were HCV seropositive. Out of 1000 patients, 735 (73.5%) were female and 265 (26.5%) were male. Mean age of the patients was 41 ± 2.33 years. Most youthful patient was 18 years of age and oldest was 89 years of age. Male and females with Hepatitis C antibodies were 122 (46.03%) and 522 (71.02%) individually. Add up to existence of gallstone in the two groups was 233 (23.3%) patients. In Group I gallstones were found in 51 (6.12%) patients and in group II (HCV+ve) in 132 (31.43%) cases. Conclusion: Hepatitis infection is positively relatedto the gallstone formation.