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H&E, TTF1, immunofluorescence staining and Digital Spatial Profiling of histologically heterogeneous lung adenocarcinoma FFPE sections.

Authors: Tavernari Daniele; Petruzzella Aaron; Dheilly Elie; Sordet-Dessimoz Jessica; Oricchio Elisa; Riggi Nicolò; Letovanec Igor; +1 Authors

H&E, TTF1, immunofluorescence staining and Digital Spatial Profiling of histologically heterogeneous lung adenocarcinoma FFPE sections.

Abstract

Please refer to the methods section of the paper for more detailed explanations. In summary: chuv_imaging_dataset_annotation.xlsx: table containing imaging assays available for each sample and histologic patterns present. he_images.zip: high-resolution images of FFPE sections stained with H&E and acquired at 20x magnification. ttf1_images.zip: high-resolution images of FFPE sections stained with TTF1 and acquired at 20x magnification. if_images.zip: high-resolution images of FFPE sections assayed with multicolor immunofluorescence (markers: CD20, CD4, CD8, CD68, Ki67) and acquired at 20x magnification. DSP_data_and_annotations.xlsx: tables containing Digital Spatial Profiling (DSP) data (in SNR) and annotations for batch1 and batch2. all_snapshots.zip: low-resolution snapshots of all images uploaded in this repository (H&E, TTF1, immunofluorescence - with all 5 colors together and with each color separately), with histologic pattern boundaries drawn on the images; immunofluorescence snapshots of DSP ROIs (batch1) and their position on each slide. Color code for histologic pattern boundaries: grey = normal, blue = lepidic, sea green = papillary, orange = acinar, red = solid. Color code for immunofluorescence channels: cyan = CD20, blue = CD4, yellow = CD8, red = CD68, green = Ki67. Color code for DSP snapshots: sea green = CD3, red = CD45, yellow = CD20, blue = DAPI

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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