Iron is an essential metal found in all living organisms including microbial, cancer and normal human cells. The main objective of the study is to analyse the iron metabolism and chelation therapy in hemosiderosis. This descriptive study was conducted in SIMS, Lahore during 2019 to 2020. This is basically a descriptive study. Hemosiderosis (iron overload) is another potential complication of transfusions. Iron is highly reactive and easily alternates between two states – iron III and iron II – in a process which results in the gain and loss of electrons, and the generation of harmful free radicals (atoms or molecules with unpaired electrons). It is concluded that nutritional iron overload requires years of supplementation to develop. Patients may take over the counterpreparations of iron supplements for a perceived health benefit and be unaware that it carries potential risks when taken for a long period of time.