Introduction: Vitamin D plays a vital role in maintaining adequate serum calcium and phosphate levels for bone mineralization and optimal skeletal health. Objectives: The main objective of this study is to analyse the association of vitamin D deficiency with ischemic cardiomyopathy. Material and methods: This cross sectional study was conducted in Central Park Medical College during 2019. All study participants underwent physical examinations, blood analysis, and echocardiographic evaluation. Venous blood samples were collected in the morning after an overnight fast (10 to 12 hours). Afterwards, the serum was separated via centrifugation and immediately transported to the laboratory for biochemical analysis. Results: The data was collected from 50 patients. There were no differences in age, gender, body mass index, and sun exposure within the groups. Biochemical parameters were not significantly different in all study participants except that patients with ICMP had low calcium levels than the control group. The mean 25(OH) D3 levels were significantly lower (14.5 ± 7.4 ng/ml vs. 28.2 ± 12 ng/ml, P = 0.001), whereas PTH (90.5 ± 28.5 pg/ml vs. 57 ± 20.2 pg/ml, P = 0.02) and NT-proBNP levels were significantly greater in patients with ICMP than controls. Conclusion: It is concluded that patients with ICMP had lower Vitamin D levels than controls, and Vitamin D deficiency had a significant correlation with cardiac function.