Every human body contains a personalized set of foreign inhabitants essential to maintaining health, yet also capable of eliciting disease. The totality of these microorganisms, their genomes and ecosystems encompasses the microbiome. The basic aim of the study is to find the role of oral microbiome in health and disease. As the correlation between the human microbiome and health becomes more evident, microbiome research is becoming central to the advancement of disease diagnostics and therapeutics as well as the development of personalized medicine. There are several ways to sequence a metagenome or segments of a metagenome, each technique serving a unique purpose. The most common techniques include 16S ribosomal RNA sequencing, pyrosequencing, and shotgun sequencing. The bacterial flora in a healthy oral cavity vs a diseased one is distinctly different, suggesting there may be a profile for a core oral microbiome of health. According to various studies, identical bacterial sequences have been discovered in the oral cavities of unrelated healthy individuals.