Indigenous medical practices have been the subject of much attention in the literature of various disciplines to date but the specificity, mode of action and clinical efficacy of most traditional plants have not been established in a manner consistent with standards of modern pharmacognosy and pharmacology. In order to find antimicrobial lead compounds and to provide scientific validation for the use of Tetrapleura tetraptera (Schum.& Thonn.) in traditional medicine . Extensive chromatographic separation of stem bark and leaf extracts led to the isolation of three distinct compounds. Anti-microbial spectrum of these obtained compounds revealed that stigma-5, 22, - diene-3-O-β-D-glucopyranoside showed activity against Staphylococcus aureus (32 mm), Streptococcus pyrogenes (30 mm) and Candida tropicalis (30 mm). It is therefore recommended for further work for a possible development of new drugs.