This data set includes x, y, and z trajectories of all GABAergic synaptic vesicles that we used for the study. These GABAergic synaptic vesicles in inhibitory presynaptic terminals of living primary hippocampal neurons were labeled by single quantum dots (QDs) conjugated with anti-VGAT antibody under electrical stimulation, and were tracked three-dimensionally by using a dual-focus imaging in real-time. Each trajectory data indicates x, y, and z positions (nanometer-scale) over time from the start of imaging to the moment of vesicle fusion. The electrical stimulation to the neurons was applied for 120 s, starting from 20 s.

{"references": ["Park, C., Chen, X., Tian, C.-L., Park, G.N., Chenouard, N., Lee, H., Yeo, X.Y., Jung, S., Bi, G.-Q., Tsien, R.W., and Park, H. (In press). Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking, Proceedings of the National Academy of Sciences of the United States of America."]}