Abstract— In this paper, we select Ginkgo biloba leaves in Taizhou as raw materials and use cellulase and pectinase to hydrolyze Ginkgo biloba leaves, and then the Ginkgo biloba leaves extract was prepared by microbial fermentation. Firstly, cellulase and pectinase were selected for single factor experiment and orthogonal experiment to determine the effect of enzyme dosage, enzymolysis time, temperature and pH value on the extraction rate of Ginkgo biloba leaves; then, microbial fermentation was used to study the effect of optimal temperature, time and pH value on the extraction rate of Ginkgo biloba leaves. The results showed that: the optimal enzyme content was 0.2%, the time of enzymolysis is 2 h, the temperature of enzymolysis was 4 oC, the pH of enzymolysis was 4.5; the optimal microorganism content of fermentation was 4%, the temperature of fermentation was 30oC, the time of fermentation was 8 D, the pH of fermentation was 5,and extraction rate was 18.56%.