Objective: The aim of this study was the Rh D negativity prevalence determination in the pregnant women of Jinnah Hospital Lahore. Material and Methods: This study was carried out at Jinnah Hospital Lahore. The data was assessed and analyzed from labor room and antenatal records. Results: Rh D negative prevalence in the pregnant women was observed as 1.4% in this research. Common most blood group was O negative (34.3%) than A negative (29.73%), B negative (21.74%) and AB blood group was found in 5.96% patients. Women parity was in the range of 0 – 18 having a mean value of (4.4 ± 3.42), including a 90% of the non-booked patients without any previous record of anti-D antibody injection. Conclusion: Negative Rhesus D alloimmunization can be prevented and it is considered as a disease that is preventable, what is required is just routine and regular check-ups, blood group Rh D negative counselling and the assured availability of the anti-D Ig in the healthcare facilities. Keywords: mutation, prophylaxis, Alloimmunization, miscarriage, placental fetal, maternal hemorrhage.