Hippocampal neurons in culture seeded on glass coverslip. This 16 bits confocal picture of hippocampal neuron in culture has been taken at 63x (1024x1024 pixels - pixel size 92,45 nm) in sequential mode with two channels : one dedicated to the PSD95 protein, and the other one to Homer protein. Those protein are expressed at glutamatergic post-synaptic sites and can be found close within Post Synaptic Density. This is a good sample to test colocalization and association analysis. The homer channel can be used both to infere neuronal shape thanks to hk-means segmentation (http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/), or to detect clusters thanks to wavelet segmentation (http://icy.bioimageanalysis.org/protocol/rainbow-spot-detector-scale-finder/). This sample image can be used with Easy SODA protocol (Standard Object Distance Analysis) on Icy software (http://icy.bioimageanalysis.org/protocol/easy-soda-2-colors-1-image/) to detect association between PSD95 and homer clusters.