The EU NSC Training Day @ NanoSafe Digital Conference was organized in two parallel sessions. The sessions in room A were providing hands-on training on nanosafety tools and gave insight into advanced cell culture models, while in room B a round table including Q&A on analytical tools, stakeholder engagement, diplomacy, regulation and risk governance sessions took place. Room A - Hands-on Training on Nanosafety Tools and Models 9:00-9:05 Welcome of the day - Martin Himly (Chair of NSC WG-A) 09:05-10:00 In silico tools for Nanosafety Research, Lang Tran (PATROLS) 10:00-12:00 Interactive training on “NanoXtract Image Analysis Tool – NanoCommons Knowledge Base”, Antreas Afantitis, Georgia Melagraki, Dieter Maier, Andreas Tsoumanis, Anastasios Papadiamantis, Iseult Lynch (NanoCommons) 12:00-13:30 Lunch break 13:30-14:30 NanoSolveIT tools for assessment of human and environmental exposure to nanomaterials Antreas Afantitis, Georgia Melagraki, Nikolaos Cheimarios, Alexander Jensen, Andreas Tsoumanis, Samuel Harisson, Pantelis Karatzas, Philip Doganis, Harry Sarimveis, Iseult Lynch (NanoSolveIT) Short break 14:30-14:40 14:40-16:40 Advanced in vitro models of the human lung, GI tract & liver for hazard characterisation, Kirsty Meldrum, Barbara Drasler, Rui-Wen He, Angela Kämpfer, Samantha Victoria Llewellyn, Marije Niemeijer (PATROLS) Short break 16:40-16:50 16:50 - 17:20 Enhanced darkfield microscopy for localization of particles in vivo/in vitro, Trine Berthing (PATROLS) Room B - NanoSafety Stakeholder engagement, Diplomacy, Regulation and Risk Governance 10:00-10:05 Welcome of the day - Stella Stoycheva (Co-Chair of NSC WG-A) 10:05-12:00 Stakeholder workshop on analytical tools, Eva Valsami-Jones (ACEnano) 12:00-13:30 Lunch break 13:30-15:00 Introduction to Nanotechnology Regulations & Risk Governance (NSC Working Group G), Steffi Friedrichs (AcumenIST), Keld Alstrup Jensen (caLIBRAte), Monique Groenewold (Gov4Nano), Thomas Kuhlbusch (NanoHarmony) Maria Dusinska (RiskGONE), Janeck Scott-Fordsmand (NanoRIGO) (NANOMET), Miguel Banares (NanoInformaTIX), Antreas Afantitis (NanoSolveIT), Iseult Lynch (NanoCommons) Short break 15:00-15:10 15:10-17:00 h Science Diplomacy: A New Way to Think About Your Role in a Community of Research, Claire Mays (SabyNA, InsSciDE), Guillermo Ort-Gils, Institute for Bioengineering of Catalonia (IBEC)