Introduction: An ethical issue can arise in any healthcare situation where profound moral questions of “right” or “wrong” underlie professional decision-making and the care of patients. Objectives: The main objective of the study is to analyse the ethical issues faced by nurses during nursing practice in Pakistan. Material and methods: This cross sectional study was conducted in Children Hospital Lahore during March 2019 to November 2019. The data was collected from 125 nurses of the hospital, which were selected randomly. The data was collected through systematically designed questionnaire. Results: The data was collected from 125 patients. The majority of respondents reported frequently or daily encountering issues related to protecting patients' rights (63.9%) and informed consent to treatment procedures (61.3%). Everyday encounters were also common with advanced care planning (41%), difficult staffing patterns (37.3%) surrogate decision-making (32.5%) and end-of life (26.2%) issues; respondents less frequently reported experiencing breaches of patient confidentiality and unethical practices. Conclusion: It is concluded that nurses were aware about their ethical responsibilities but were often unable to practice them.