This is a dataset containing fragments of internal transcribed spacer 2 (ITS2) extracted from Bistorta vivipara root-associated fungi, sampled from snow fence experiment in Adventdalen, Svalbard. This dataset was used in Wutkowska et al., 2020, Can root-associated fungi mediate the impact of abiotic conditions on the growth of a High Arctic herb?Can root-associated fungi mediate the impact of abiotic conditions on the growth of a High Arctic herb? [available at biorXiv.org, DOI: 10.1101/2020.06.20.157099] Now the manuscript is available as a peer-reviewed paper: Wutkowska, Magdalena, Dorothee Ehrich, Sunil Mundra, Anna Vader, and Pernille Bronken Eidesen. 2021. ‘Can Root-Associated Fungi Mediate the Impact of Abiotic Conditions on the Growth of a High Arctic Herb?’ Soil Biology and Biochemistry 159:108284. doi: 10.1016/j.soilbio.2021.108284. All other corresponding data are available here: https://github.com/magdawutkowska/bistorta