Introduction: The commonly used local anesthetic agent has been lignocaine. Lignocaine acts by blocking depolarization at nerve endings, inducing anesthesia locally. Objectives: The basic aim of the study is to find the level of blood glucose concentration in diabetic patients undergoing tooth extraction by using local anesthesia. Material and methods: This cross sectional study was conducted at DHQ hospital, Pakpattan during October 2018 to June 2019. Patients included were in the age group of 25–55 years and had a necessity for extraction. Results: There is no statistically significant difference between the groups, regarding the evaluation period (p> 0.05). However, when comparing the periods statistically significant differences were observed (p <0.05) for T2 and T3 values for group. Conclusion: It is concluded that periodontal disease is the main oral clinical manifestation in diabetic patients. Poorly controlled diabetes could lead to complications that may even be life- threatening.