Abstract— Thirty five E.coli isolates were collected from patient suffering from long term urinary tract catheters and urinary tract infection for both genera with age ranging between (11-60) years. The isolates were identified according to cultural, microbiological, biochemical testes. Detection bacteriocin production by E. coli was showed only (5) isolates of E coli produced bacteriocin. To esteemed effect of P. cubeba fruits extract on bacteriocin production from E. coli exposed to this extract, the results showed did not changing in bacteriocin production but the extract caused changes in the antimicrobial activity between bacteriocin produced before and after treated bacteria with P. cubeba fruits extract. In the case of bacteria cultured without the extract, protein concentration of partially purified bacteriocin (by ammonium sulphat) was (9.21mg/ml) while bacteriocin protein concentration of bacteria grown with the extract was (10.6mg/ml). The statistical analysis of result did not show significant differences and “p value” was 0.177. SDS PAGE reveal that bacteriocin have molecular weight (25KDa) and identified as Colicin.