Background: Diabetic foot Ulcer is one of most significant and devastating complications of Diabetes. To study risk factors for multi-drug resistant organisms in Diabetic foot ulcer; impact of Glycemic control on wound healing using Chi square test, student’s t test logistic regression analysis. Methods: In 100 patients hospitalized, microbiological specimens taken on admission. Potential risk factors for MDRO-positive specimens were examined using univariate analyses, logistic regression for MDRO presence and wound healing time. Prospective follow-up data from patients used to evaluate the influence of MDRO infection& glycemic control on time to healing. Results: MDRO were isolated in 75 of 100 patients. Poor glycemic control, previous hospitalization, history of amputation, history of antibiotic use, ulcer size, necrotic ulcer, recurrent ulcers, higher grade of ulcer and polymicrobial culture were associated with MDRO infected foot ulcers (p <0.1). MDRO has no impact on wound healing. Logistic regression analysis indicated higher Grade of ulcer, poor glycemic control significantly delayed wound healing. Conclusions: The prevalence of MDRO is alarmingly high. Higher grade ulcers & recurrent ulcers are more prone to acquire MDROs. Positive MDRO status is not associated with wound healing. Higher grade of ulcer & poor glycemic control delays healing of foot ulcer.