Data on flowering time and grain yield for each of the 1,093 barley accessions, including the 895 genotyped accessions, were collected through field experimentsover three years in, 2015, 2016 and 2017, at five locations (Esperance, Geraldton, Katanning, Merredin, and South Perth) in Western Australia that significantly differ in terms of rainfall and temperature during the growing seasons. All regional field trials were planted in a randomised, complete-block design with plots of 1.1 by 3.0m2laid out in a row-column format. Field trials at South Perth were conducted using a hill plot technique with a 40-cm distance within and between rows. Seven control varieties were used for a spatial adjustment of the experimental data. In each plot of each experiment in the study, measurements were taken to determine the flowering time and grain yield. Awn emergence, defined as the number of days from sowing to the first awn of ~2 cm emerging above the flag leaf (Z49) was recorded as an equivalent to flowering time, and Z91 as days from sowing to maturity. werealso recorded when possible. Grain yield was estimated by harvesting all plant material from each plot to separate the grain from determining grain mass and from estimating the grain yield (kg.ha-1).