Abstract The search for life in the universe mainly uses modern Earth as a template. However, we know that Earth’s atmospheric composition changed significantly through its geological evolution. Recent discoveries show that transiting, potentially Earthlike, exoplanets orbit a wide range of host stars, which strongly influence their atmospheric composition and remotely detectable spectra. Thus, a database for transiting terrestrial exoplanets around different host stars at different geological times is a crucial missing ingredient to support observational searches for signs of life in exoplanet atmospheres. Here, we present the first high-resolution transmission spectra database for Earthlike planets, orbiting a wide range of host stars, throughout four representative stages of Earth’s history. These correspond to a prebiotic high-CO2 world—about 3.9 billion years ago in Earth’s history—and three epochs through the increase in oxygen from 0.2% to modern atmospheric levels of 21%. We demonstrate that the spectral biosignature pairs O2+CH4 and O3+CH4 in the atmosphere of a transiting Earthlike planet would show a remote observer that a biosphere exists for oxygen concentrations of about 1% of modern Earth’s—corresponding to about 1–2 billion years ago in Earth’s history—for all host stars. The full model and high-resolution transmission spectra database, covering 0.4–20 μm, for transiting exoplanets—from young prebiotic worlds to modern Earth analogs orbiting a wide range of host stars—is available online. It is a tool to plan and optimize our observation strategy, train retrieval methods, and interpret upcoming observations with ground- and space-based telescopes.