Vitellogenin (VTG) is known to be a female-specific protein and a precursor form of egg yolk. Threevitellogenin,Vtg I,Vtg II and Vtg III have been identified in chicken.Vitellinogen precursors supply the major egg yolk proteins which are a source of nutrients during early development of egg-laying (oviparous) vertebrates and invertebrates. A complete structural analysis and 3-D modelling of Vitellogenin protein of Chicken (Target), has been already done. The 1852 amino acid sequence of the Vitellogenin protein (Gallus gallus) was retrieved from NCBI database. OOPS model of Multiple EM for Motif Elicitation (MEME) online software package was used to carry out this analysis. Total fifteen amino acid sequences were taken for motif analysis.According to our results, Vitellogenin sequences, contains three motifs. We analyze that all fifteen sequences includes all threemotifs but their start points are different. Motif investigationsuggeststhat all selected VTG sequences from different resources have common conserved patterns.