The present work emphasized on developing a simple HPTLC fingerprint profile of ethanolic extracts of Hemidesmus indicus roots and stems of Tinospora cordifolia as well as their combine mixture for separation of active compounds and their qualitative study Ethanolic extracts of the stem and roots parts were prepared by Soxhlet extraction. CAMAG HPTLC system with the help of aluminum thin layer chromatography (TLC) plate precoated with silica gel 60 F 245 Linomat applicator, TLC scanner 3 and win CATS software were used, ethanolic extracts of different samples at different concentrations were developed in suitable solvent system Toluene: ethyl acetate: formic acid (10:3:1) and scanned by densitometric scanner in UV light reflectance- absorbance mode regulated by wins cats software. The plate was further placed in photodocumentation chamber (CAMAG REPROSTAR3) and images were captured at UV 366nm and 254nm, it was further subjected to HPTLC fingerprinting where Rf value, Area Under Curve and percentage area were calculated. HPTLC profile displayed several peaks with distinguished Rf values.